Methods and apparatus for the selection and/or processing of...

B - Operations – Transporting – 03 – C

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B03C 5/00 (2006.01) B03C 5/02 (2006.01)

Patent

CA 2649017

Methods and apparatus for the selection or processing of particles sensitive to the application of an external stimulus, in which is produced, by applying the external stimulus, the rupture/lysis of at least one selected particle or the fusion of first and second selected particles, by means of the organisation of the particles (CELL) using a first field of force (FMAN) by selectively energising electrodes (EL) of an array of selectable electrodes having dimensions comparable to or smaller than those of the particles, applying to the electrodes a first configuration (PMAN) of stresses; and by applying to the electrodes a second configuration (PZAP) of stresses, so as to create a second field of force (FZAP), located substantially close to at least one selected particle to be lysated (CELL) or to a pair of first and second particles to be fused and such as to produce the application of a stimulus suited to produce their lysis or fusion.

L'invention concerne des procédés et des appareils pour la sélection ou le traitement de particules sensibles à l'application d'un stimulus externe, lequel stimulus externe entraîne, lors de son application, la rupture ou lyse d'au moins une particule choisie, ou la fusion d'une première et d'une seconde particule choisie, les particules (CELLULE) s'organisant sous l'effet d'un premier champ de force (FMAN) appliqué par l'activation sélective des électrodes (EL) d'un réseau d'électrodes sélectionnables, dont les dimensions sont inférieures ou comparables à celles des particules, une première configuration (PMAN) de contraintes étant appliquée aux électrodes, et une seconde configuration (PZAP) de contraintes étant appliquée aux électrodes afin de créer un second champ de force (FZAP), situé sensiblement près d'au moins une particule sélectionnée pour être lysée (CELLULE) ou d'une paire de première et seconde particule à fusionner, de manière à entraîner l'application d'un stimulus apte à produire la lyse ou la fusion de ces dernières.

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