Methods and apparatuses for purification

Details Methods and apparatuses for purification Methods and apparatuses for purification

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) B01D 15/00 (2006.01) B01D 15/36 (2006.01) B01D 15/38 (2006.01) B01J 41/04 (2006.01) C07K 14/205 (2006.01) C07K 14/21 (2006.01) C07K 14/245 (2006.01) C07K 14/31 (2006.01) C07K 14/315 (2006.01) C12N 1/06 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 9/06 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/48 (2006.01) C12N 9/78 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 15/62 (2006.01) G01N 30/88 (2006.01)

Patent

CA 2460819

Proteins can be purified and concentrated from crude cell lysates using high- throughput, automated process control systems that use software application programs to instruct one or more processors, controllers, and actuators to control and/or monitor the operations of filtration, purification, and concentration modules in such systems. The high-throughput production of concentrated proteins can be enabled by filtering the lysates and using compressed, non-reactive gas to concentrate the purified proteins, rather than using more complex centrifugation techniques.

Des protéines peuvent être purifiées et concentrées à partir de lysats de cellules brutes à l'aide de systèmes à haut rendement et à commande de processus automatisée. Ces systèmes reposent sur l'utilisation de programmes d'application pour donner des instructions à un ou plusieurs processeurs, dispositifs de commande et actionneurs destinés à commander et / ou surveiller les opérations de modules de filtration, de purification et de concentration dans des systèmes de ce type. La production à haut rendement de protéines concentrées repose sur la filtration des lysats et l'utilisation de gaz comprimé non réactif pour concentrer les protéines purifiées, plutôt que sur l'utilisation de techniques de centrifugation plus complexes.

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