Methods and applications of biomarker profiles in the...

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2525725

The present invention provides biomolecules and the use of these biomolecules for the differential diagnosis of a breast cancer and/or a non-malignant disease of the breast. In particular the present invention provides methods for detecting biomolecules within a test sample as well as a database comprising of mass profiles of biomolecules specific for healthy subjects, subjects having a precancerous lesion of the breast, subjects having a breast cancer or a metastasised breast cancer or subjects having a non-malignant disease of the breast. Furthermore, the present invention provides methods for the characterization of said biomolecules using gas phase ion spectrometry. In addition, the present invention provides methods for the identification of said biomolecules provided that they are proteins or polypeptides. The invention further provides kits for the differential diagnosis of a non- malignant disease of the breast or breast cancer.

L'invention concerne des biomolécules et leur utilisation afin d'effectuer un diagnostic différentiel d'un cancer du sein et/ou d'une maladie bénigne du sein. D'une manière plus spécifique, l'invention concerne des méthodes permettant de détecter des biomolécules dans un échantillon d'essai ainsi que dans une base de données comprenant des profils de masse de biomolécules spécifiques de sujets sains, de sujets souffrant d'une lésion précancéreuse du sein, de sujets souffrant d'un cancer du sein ou d'un cancer du sein métastasé ou de sujets souffrant d'une maladie bénigne du sein. L'invention concerne également des méthodes permettant de caractériser lesdites biomolécules au moyen de la spectrométrie d'ions en phase gazeuse. L'invention concerne en outre des méthodes permettant d'identifier lesdites biomolécules à la condition qu'elles soient des protéines ou des polypeptides. L'invention concerne enfin des trousses permettant d'effectuer un diagnostic différentiel d'une maladie bénigne du sein et/ou d'un cancer du sein.

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