Methods and associated reagents for detecting modulators of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2191189

The present invention provides DNA constructs that contain oligonucleotide sequences comprising DNA regulatory elements of the general sequence TTNXAA that bind activated transcriptional regulatory proteins in response to signaling molecules, such as cytokines, an operably linked promoter and operably linked heterologous gene. The present invention also provides host cells transfected with such DNA constructs, as well as methods for measuring the ability of compounds to act as agonists and antagonists of gene transcription utilizing these DNA constructs and transfected host cells.

La présente invention concerne des ADN de recombinaison contenant des séquences oligonucléotidiques comprenant des structures régulatrices de l'ADN ayant la séquence générale TTN¿X?AA fixant des protéines impliquées dans le contrôle de la transcription et activées par des molécules signal, comme par exemple les cytokines, un opérateur lié de manière à être fonctionnel et un gène hétérologue lié de manière à être fonctionnel. La présente invention concerne également des cellules hôtes transformées par ces ADN de recombinaison, ainsi que de procédés pour mesurer le pouvoir agoniste ou antagoniste de divers composés dans la transcription de gènes, lorsqu'on utilise ces ADN de recombinaison de ces cellules hôtes transformées.

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