Methods and composition to generate unique sequence dna...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2623405

The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.

L'invention concerne de manière générale le domaine de l'identification de séquences, de gènes ou de chromosomes d'ADN. L'invention concerne également des procédés et une composition destinés à obtenir des sondes d'ADN à séquence unique. La composition de l'invention comprend des séquences uniques contenant de l'ADN double brin à partir desquelles les séquences répétitives sont éliminées selon le procédé décrit dans l'invention. Cette invention concerne également la conservation de cellules qui ont été identifiées après sélection immunomagnétique et étiquetage fluorescent afin d'interroger les cellules d'intérêt. En outre, l'invention concerne l'analyse génétique de cellules qui ont été identifiées après sélection immunomagnétique et étiquetage fluorescent.

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