C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/67 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2703847
The invention relates to a tRNA synthetase capable of binding N~-acetyl lysine, hi particular, the invention relates to a tRNA synthetase capable of binding N~- acetyl lysine wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of MbPy1RS. The invention also relates to a method of making a polypeptide comprising N~ -acetyl lysine comprising arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, wherein said translation is carried out in the presence of a polypeptide according to any of claims 1 to 11 and in the presence of tRNA capable of being charged with N~-acetyl lysine, and in the presence of N~-acetyl lysine.
L'invention porte sur une ARNt synthétase capable de se lier à la N?-acétyl lysine. En particulier, l'invention porte sur une ARNt synthétase capable de se lier à la N?-acétyl lysine, la synthétase comprenant un polypeptide ayant au moins 90 % d'identité de séquence avec la séquence d'acides aminés de MbPy1RS. L'invention porte également sur un procédé de fabrication d'un polypeptide comprenant de la N?-acétyl lysine, le procédé comprenant l'arrangement pour la traduction d'un ARN codant pour ledit polypeptide, ledit ARN comprenant un codon UAG, ladite traduction étant réalisée en présence d'un polypeptide selon l'une quelconque des revendications 1 à 11 et en présence d'un ARNt capable d'être chargé par la N?-acétyl lysine, et en présence de N?-acétyl lysine.
Chin Jason
Neumann Heinz
Borden Ladner Gervais Llp
Medical Research Council
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