Methods and compositions for designing nucleic acid...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2605939

The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.

L'invention concerne des procédés de conception de molécules d'acides nucléiques permettant d'obtenir une expression améliorée des polypeptides codés dans des plantes. Dans ces procédés, des fréquences d'utilisation de codons sont biaisées vers des fréquences d'utilisation de codons d'un virus de plante, d'un groupe de virus de plante ou d'un sous-ensemble de molécules d'acides nucléiques issues de ceux-ci. Dans des modes de réalisation préférés, le polypeptide codé modifie le phénotype de la plante. L'invention concerne également des molécules d'acides nucléiques codant des polypeptides insecticides, ces molécules d'acides nucléiques ayant été conçues pour présenter un biais de codons de virus de plante. L'invention concerne encore des plantes transgéniques et leur descendance, présentant une expression accrue de polypeptides insecticides pour une résistance améliorée aux insectes et autres parasites nuisibles aux plantes à valeur agricole.

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