Methods and compositions for detecting binding of ligand...

Details Methods and compositions for detecting binding of ligand... Methods and compositions for detecting binding of ligand...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 13/04 (2006.01) C07H 21/00 (2006.01) C08G 73/00 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2243989

Methods are provided for detecting the binding of a first member to a second member of a ligand pair, comprising the steps of (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry, or potentiometry; (b) separating bound first and second members from unbound members; (c) cleaving the tag from the tagged first member; and (d) detecting the tag by non-fluorescent spectrometry, or potentiometry, and therefrom detecting the binding of the first member to the second member.

L'invention concerne des procédés servant à détecter la fixation d'un premier élément à un deuxième élément d'une paire de ligands et consistant (a) à combiner un ensemble de premiers éléments marqués avec un spécimen biologique pouvant contenir un ou plusieurs deuxièmes éléments dans des conditions et pendant une durée suffisantes pour permettre la fixation d'un premier élément à un deuxième élément, ledit marqueur étant corrélé avec un premier élément en particulier et détecté par spectrométrie non fluorescente ou potentiométrie, (b) à séparer le premier et le deuxième éléments fixés d'éléments non fixés, (c) à séparer le marqueur du premier élément marqué et (d) à détecter le marqueur par spectrométrie non fluorescente ou potentiométrie et, de ce fait, détecter la fixation du premier élément au deuxième élément.

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