Methods and compositions for detecting rhinoviruses

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2576955

The invention provides methods and compositions for rapid, sensitive and highly specific nucleic acid-based (e.g., DNA based) detection of human rhinovirus (HRV) in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved 5~ untranslated region of the HRV genome. The invention also features compositions, including primers, probes and kits, for use in the methods of the invention.

L'invention concerne des méthodes et des compositions pour la détection basée sur un acide nucléique, rapide, sensible et hautement spécifique (par ex., basée sur l'ADN) de rhinovirus humain (HRV) dans un échantillon. En général, les méthodes impliquent la détection d'un acide nucléique cible présentant une séquence cible d'une région 5' non traduite, conservée, du génome du HRV. L'invention concerne également des compositions, y compris des amorces, des sondes et des trousses, destinés à être utilisés dans les méthodes de l'invention.

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