C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) G01N 21/78 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2489922
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
Cette invention concerne des méthodes, compositions et articles de fabrication s'utilisant pour la recherche d'un polynucléotide cible dans un échantillon. Un échantillon dont on pense qu'il renferme le polynucléotide cible est mis en contact avec un mutlichromophore polycationique et un acide nucléique peptidique (PNA) de détection qui est complémentaire dudit polynucléotide cible. Ledit PNA comprend un chromophore de signalisation qui absorbe l'énergie dégagée par le multichromophore excité et émet de la lumière en présence du polynucléotide cible. Ces méthode peuvent être utilisées pour des analytes multiples simultanément. L'invention porte également sur des trousses contenant des réactifs pour l'application de telles méthodes.
Bazan Guillermo C.
Gaylord Brent S.
Gowling Lafleur Henderson Llp
The Regents Of The University Of California
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