C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2480673
The present invention provides novel solution phase hybridization-based methods for detecting and quantitating nucleic acid analytes. Methods comprising use of novel capture polymers and/or signaling systems are provided. Use of these novel capture polymers and/or signaling systems provides significant improvements in signal to noise ratio, specificity, sensitivity and ease of development and use as compared to existing solution phase nucleic acid detection and quantitation methods. The invention further provides compositions, kits and articles of manufacture for practicing methods of the present invention.
L'invention porte sur de nouveaux procédés à base d'hybridation de détection et quantification d'analytes d'acides nucléiques en phase soluble comprenant l'utilisation de nouveaux polymères de capture et/ou systèmes de signalisation. L'utilisation de ces nouveaux polymères apporte une amélioration significative du rapport signal/bruit, de la spécificité, de la sensibilité, et de la facilité de développement et d'utilisation, par rapport aux procédés actuels de détection et quantification d'acides nucléiques en phase soluble. L'invention porte en outre sur des compositions, des trousses et des articles manufacturés de mise en pratique des procédés de l'invention.
Billeci Todd
Stephan Jean-Philippe F.
Tsai Siao Ping
Wong Wai Lee Tan
Genentech Inc.
Smart & Biggar
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