Methods and compositions for detection of complement fixing...

G - Physics – 01 – N

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G01N 33/564 (2006.01)

Patent

CA 2740192

The invention provides methods for sensitive and specific detection of complement fixing antibodies in a biologi-cal sample using complement factor C1q, including autologous complement factor C1q present in the biological sample and a de-tectably labeled antibody that binds the autologous complement factor C1q, exogenous human complement factor C1q and a de-tectably labeled antibody that binds the exogenous human complement factor C1q, detectably labeled exogenous human comple-ment factor C1q, or a combination of autologous complement factor C1q and exogenous human complement factor C1q. The in-vention also features kits, systems, and devices for use in the methods of the invention.

La présente invention concerne des procédés pour la détection sensible et spécifique danticorps fixant le complément dans un échantillon biologique en utilisant le facteur de complément C1q, comprenant le facteur de complément autologue C1q présent dans léchantillon biologique et un anticorps marqué de manière détectable qui se lie au facteur de complément autologue C1q, le facteur de complément humain exogène C1q et un anticorps marqué de manière détectable qui se lie au facteur de complément humain exogène C1q, le facteur de complément humain exogène C1q marqué de manière détectable, ou une combinaison de facteur de complément autologue C1q et de facteur de complément humain exogène C1q. Linvention concerne en outre des kits, des systèmes, et des dispositifs pour utilisation dans les procédés de linvention.

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