Methods and compositions for detection of microbial...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/37 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2555209

Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions are provided. A novel cause of aseptic peritonitis is provided--aseptic peritonitis associated with gram positive microbial contamination of a dialysis solution. Peptidoglycan is a major component of a gram positive bacterial cell wall and thus can serve as a marker for gram positive bacteria. In this regard, testing for peptidoglycans can be utilized to effectively prevent peritonitis in patients that use the peritoneal dialysis solutions, such as peritoneal dialysis solutions that contain a glucose polymer including an icodextrin and the like.

L'invention concerne des procédés et des compositions qui permettent de détecter des contaminants microbiens dans des solutions de dialyse péritonéale. Il a été découvert une nouvelle cause de péritonite aseptique, la péritonite aseptique associée à la contamination d'une solution de dialyse par des microbes gram-positifs. Le peptidoglycan est un composant essentiel de la paroi cellulaire des bactéries gram-positives et peut, par conséquent, être utilisé comme marqueur des bactéries gram-positives. A cet égard, on peut utiliser la recherche des peptidoglycans pour prévenir de manière efficace la péritonite chez des patients utilisant des solutions de dialyse péritonéale, telles que des solutions de dialyse péritonéale contenant un polymère du glucose comprenant une icodextrine et analogues.

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