Methods and compositions for detection or quantification of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2300940

The present invention provides a method for detecting a target nucleic acid species using an array of probes affixed to a substrate and a plurality of labeled probes. The invention also relates to oligonucleotide probes attached to discrete particles wherein the particles can be grouped into a plurality of sets based on a physical property. A different probe is attached to the discrete particles of each set, and the identity of the probe is determined by identifying the discrete particles from their physical property. The invention further relates to methods using agents which destabilize the binding of complementary polynucleotide strands (decrease the binding energy) or increase stability of binding between complementary polynucleotide strands (increase the binding energy). The figure is an illustration of an apparatus for mass producing probe arrays.

L'invention porte sur un procédé de détection d'espèces d'acides nucléiques cibles recourant à une série de sondes fixées à un substrat et à un ensemble de sondes marquées. L'invention porte également sur des sondes d'oligonucléotides fixées à des particules distinctes pouvant être groupées en plusieurs ensembles en fonction d'une caractéristique physique, une sonde différente étant fixée à chacune des particules distinctes de chacun des ensemble, et l'identité des sondes étant déterminée par l'identification des particules distinctes en fonction de leur caractéristique physique. L'invention porte en outre sur des procédés utilisant des agents déstabilisant la fixation de brins complémentaires de polynucléotides (décroissance de l'énergie de liaison), ou accroissant la stabilité des liaisons entre brins complémentaires de polynucléotides (croissance de l'énergie de liaison). La figure (1) est une illustration d'une appareil de production en masse d'ensembles de soudes.

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