Methods and compositions for determining latent viral load

Details Methods and compositions for determining latent viral load Methods and compositions for determining latent viral load

G - Physics

01

N

G01N 33/569 (2006.01) C12Q 1/70 (2006.01) G01N 33/533 (2006.01) G01N 33/537 (2006.01) G01N 33/541 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2371661

The present invention relates to a new HIV stows of a patient called "latent viral load". To measure the "latent viral load." in accordance with a preferred embodiment of the present invention, a population of sample cells is obtained from a desired source, such as an infected patient. The sample cell population is depleted of overtly infected cells and cells harboring active virus, to produce a subset of "resting cells" comprising uninfected and latently- infected cells. This subset is treated with an agent and/or condition that activated the latent virus in the host cell genome and results in a productive infection. The thus-produced infection reflects the "latent viral load" of the host because it reveals the presence of quiescent virus in cells. The latent viral load is useful ie assessing a patient's disease status and the efficacy of hight active antiretroviral therapy and other treatment protocols.

L'invention concerne un nouvel état du VIH d'un patient, appelé "charge virale latente". Afin de mesurer cette charge, un procédé de réalisation préféré de l'invention comprend les étapes consistant à obtenir une population de cellules échantillons à partir d'une source voulue, telle qu'un patient infecté, à appauvrir cette population de cellules échantillons en lui enlevant les cellules infectées de manière patente et les cellules hébergeant le virus actif, afin d'obtenir un sous-ensemble de cellules quiescentes comprenant des cellules non infectées et des cellules infectées de manière latente, à traiter ce sous-ensemble avec un agent et/ou une condition activant le virus latent dans le génome cellulaire hôte et provoquant l'infection productive qui va refléter la charge virale latente de l'hôte étant donné qu'elle révèle la présence du virus à l'état quiescent dans les cellules, et enfin à utiliser la charge virale latente pour déterminer l'état de la maladie du patient et l'efficacité d'une thérapie antirétrovirale très active et d'autres protocoles de traitement.

Affiliated with

Also associated with

Rating

Methods and compositions for determining latent viral load does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for determining latent viral load, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for determining latent viral load will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2064342