Methods and compositions for determining the purity of...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/18 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/552 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2402131

This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.

Cette invention concerne un anticorps qui se lie spécifiquement à un oligomère synthétique (tel qu'un oligonucléotide ou un oligopeptide) auquel est lié par covalence un groupe de protection organique, ledit anticorps ne se liant pas à cet oligomère synthétique lorsque le groupe de protection organique n'y est pas lié par covalence. L'invention porte également sur des procédé d'obtention et d'utilisation de tels anticorps, ainsi que sur des cellules intervenant dans la fabrication des ces anticorps et sur des articles qui portent des oligomères immobilisés pouvant s'utiliser dans le cadre de méthodes d'analyse avec de tels anticorps.

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