Methods and compositions for diagnosing and treating...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2384855

The present invention relates to improved diagnostic methods for early detection of a risk for developing an arthritic disorder in humans, and screening assays for therapeutic agents useful in the treatment of arthritic disorders, by comparing the levels of one or more indicators of altered mitochondrial function. Indicators of altered mitochondrial function include enzymes such as mitochondrial enzymes and ATP biosynthesis factors. Other indicators of altered mitochondrial function include mitochondrial mass, mitochondrial number and mitochondrial DNA content, cellular responses to elevated intracellular calcium and to apoptogens, and free radical production. Methods of treating, and of stratifying, human patients as such methods relate to disclosed indicators of altered mitchondrial function are also provided.

La présente invention concerne des procédés diagnostiques améliorés destinés à la détection précoce des risques que présente un patient humain de développer un trouble arthritique, ainsi que des dosages destinés au criblage d'agents thérapeutiques, utiles dans le traitement des troubles arthritiques, par la comparaison des teneurs d'un ou de plusieurs indicateurs de modification de la fonction mitochondriale. Les indicateurs de modification de la fonction mitochondriale comprennent des enzymes et des facteurs de biosynthèse de l'ATP. D'autres indicateurs de modification de la fonction mitochondriale comprennent la masse mitochondriale, le nombre des mitochondries et la teneur en ADNmt ainsi que la réponse cellulaire au calcium intracellulaire élevé et aux apoptogènes et la production de radicaux libres. L'invention concerne aussi des procédés de traitement et de classification des patients humains, ces procédés se rapportant aux indicateurs de modification de la fonction mitochondriale décrits dans l'invention.

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