C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2377938
The present invention is directed to methods and compositions for DNA subcloning using bacterial recombinase-mediated homologous recombination. The invention relates to methods for cloning, compositions comprising polynucleotides useful as cloning vectors, cells comprising such polynucleotide compositions, and kits useful for cloning mediated by bacterial recombinases, such as RecE/T and Red.alpha./.beta..
Cette invention se rapporte à des procédés et à des compositions pour le sous-clonage d'ADN utilisant la recombinaison homologue induite par des recombinases bactériennes. Cette invention se rapporte à des procédés de clonage, à des conditions comprenant des polynucléotides utiles comme vecteurs de clonage, à des cellules comprenant ces compositions de polynucléotides, et à des kits utiles dans des opérations de clonage induit par des recombinases bactériennes, telles que RecE/T et Red.alpha./.beta..
Muyrers Joep Pieter Paul
Stewart A. Francis
Zhang Youming
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
The European Molecular Biology Laboratory
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