Methods and compositions for evaluating graft survival in a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2601623

Methods are provided for evaluating a subject for graft survival, e.g., in terms of predicting graft survival, identifying the presence of a deleterious graft condition, such as CAN and DT, identifying the severity and class of acute rejection, etc, in a subject are provided. In practicing the subject methods, the expression of at least one gene in a sample from the subject, e.g., a blood or biopsy sample, is assayed, e.g., at the nucleic acid and/or protein level, to evaluate the subject. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications.

L'invention concerne des procédés permettant d'évaluer la survie d'un greffon chez un sujet, par exemple, en termes de prévision de la survie du greffon, d'identification de la présence d'un état délétère du greffon, tel que CAN et DT, d'identification de la gravité et de la classe du rejet aigu etc.. La mise en oeuvre des procédés selon l'invention permet d'analyser l'expression d'au moins un gène dans un échantillon prélevé chez le sujet, par exemple, un échantillon de sang ou de biopsie, au niveau des acides nucléiques et/ou des protéines, aux fins d'évaluation du sujet. L'invention concerne également des compositions, des systèmes et des kits à utiliser dans la mise en oeuvre des procédés selon l'invention. Les procédés et compositions sont utiles dans une palettes d'applications.

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