Methods and compositions for expressing negative-sense viral...

Details Methods and compositions for expressing negative-sense viral... Methods and compositions for expressing negative-sense viral...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) A61K 39/145 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/33 (2006.01) C12N 15/44 (2006.01) C12N 15/86 (2006.01) C12Q 1/70 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2613284

The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

L'invention se rapporte à de nouvelles séquences d'acide nucléique canines régulatrices pol 1 utiles pour exprimer des séquences d'acide nucléique dans des cellules canines telles que des cellules MDCK. L'invention concerne également des vecteurs et des cellules d'expression renfermant lesdits acides nucléiques ainsi que des procédés d'utilisation desdits acides nucléiques pour préparer des influenzavirus, y compris des influenzavirus infectieux.

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