Methods and compositions for expression of transgenes in plants

Details Methods and compositions for expression of transgenes in plants Methods and compositions for expression of transgenes in plants

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2328129

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize- derived transgenes are desirable for expression in maize in that homology- based gene silencing can limit or effectively eliminate transgene expression.

On décrit des procédés et des compositions qu'on utilise pour exprimer des transgènes dans des plantes monocotylédones y compris dans du maïs. Dans cette invention il est inutile de rendre les gènes silencieux du fait qu'on utilise des séquences monocotylédones-homologues provenant de plantes du genre Coix pour la transformation. Dans ces séquences de transgènes se trouvent inclus des promoteurs, des activateurs, des séquences de codage et des terminateurs Coix. Il est intéressant de trouver des substituants appropriés aux transgènes dérivés du maïs pour l'expression dans le maïs étant donné que la mise au silence des gènes fondée sur l'homologie peut limiter ou pratiquement éliminer l'expression des transgènes.

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