Methods and compositions for extracting proteins from cells

Details Methods and compositions for extracting proteins from cells Methods and compositions for extracting proteins from cells

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) C07K 1/14 (2006.01) C07K 1/20 (2006.01) C12N 1/00 (2006.01) C12N 1/14 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2434844

The present invention relates to a process of releasing a protein, recombinant or otherwise, from a cell. The process of the present invention involves contacting a host cell containing a protein of interest with a solution comprising one or more detergents and one or more reducing agents. The methods of the invention are particularly suitable to large scale production of recombinant products.

L'invention concerne un procédé permettant de séparer une protéine recombinée ou une autre protéine d'une cellule. Ce procédé consiste à faire entrer en contact une cellule hôte contenant une protéine choisie avec une solution comprenant un ou plusieurs détergents et un ou plusieurs agents réducteurs. Les procédés décrits conviennent en particulier pour la production à grande échelle de produits de recombinaison.

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