Methods and compositions for gene, tumor, and viral...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/83 (2006.01) A61K 31/70 (2006.01) A61K 35/30 (2006.01) A61K 38/16 (2006.01) A61K 39/245 (2006.01) A61K 48/00 (2006.01) C07K 14/035 (2006.01) C12N 5/00 (2006.01) C12N 15/869 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2132976

The present invention relates to methods of treatment of programmed cell death (apoptosis) through the use of the HSV-1 gene .gamma.1 34.5 or the product of its expression, ICP34.5. The gene and its expression have been demonstrated to be required for HSV-1 neurovirulence, and in particular, to act as an inhibitor of neuronal programmed cell death which allows for viral replication. Use of the gene therapy, or the protein itself, can be expected to result in inhibition of programmed cell death in various neurodegenerative diseases. This invention also relates to novel vectors for gene therapy, including modified herpes virus. Methods are presented for conducting assays for substances capable of mimicing, potentiating or inhibiting the expression of .gamma.1 34.5 or the activity of ICP34.5. Also, methods are disclosed for the treatment of tumorogenic diseases, including cancer, and for treatment of herpes and other viral infections using inhibitors of .gamma.1 34.5 expression or ICP34.5 activity.

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