Methods and compositions for generating and amplifying dna...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2559209

The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In several aspects of the present invention, there are methods based on methylation- dependent enrichment or depletion of genomic DNA isolated from cellular and cell-free sources. In additional embodiments, there are methods and compositions for single-step high throughput preparations of Methylome libraries.

La présente invention a trait à une variété de procédés et de compositions pour l'obtention d'information épigénétique, telle que des configuration de méthylation d'ADN, par la présentation de la préparation, l'amplification et l'analyse de bibliothèques d'ensembles de méthylation complète de l'ADN. Dans divers aspects, la présente invention a trait à des procédés basés sur l'enrichissement ou l'appauvrissement dépendant de la méthylation de l'ADN génomique isolé de sources cellulaires et acellulaires. Dans d'autres modes de réalisation, l'invention a trait à des procédés et des compositions pour des préparations à haut rendement en une seule étape de bibliothèques d'ensembles de méthylation complète de l'ADN.

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