C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A61K 35/12 (2006.01) A61K 39/00 (2006.01) A61K 39/39 (2006.01) A61K 48/00 (2006.01) C07K 14/035 (2006.01) C07K 14/11 (2006.01) C07K 14/34 (2006.01) C07K 14/55 (2006.01) C07K 14/57 (2006.01) C12N 5/10 (2006.01)
Patent
CA 2091346
The invention provides a preparation of tumor cell lines containing an exogenous gene encoding a polypeptide that potentiates the immune response to the tumor, for example, interleukin-2. The invention includes methods for cancer therapy by immunization with the immunopotentiating tumor cell. The invention also provides methodology whereby one can selectively ablate in vitro and in vivo genetically altered cells. A preferred embodiment of this aspect of the invention includes a cell containing a first exogenous gene encoding an immunopatentiating polypeptide and a second exogenous gene encoding a "lethal" or "suicide" polypepticide, preferably under the control of an inducible promoter. Introduction of the second exogenous gene confers the ability to selectively kill the tumor cell by inducing a promoter in operative linkage to the gene encoding the lethal polypeptide to initiate transcription of the polypeptide. Diphtheria toxin or the thymidine kinase of Herpes simplex virus are exemplary lethal genes. An exemplary promoter employed is tho 6-16 promoter, which is inducible with low levels of interferon. Methods for using the cells in cancer therapy are also provided:
Chernajovsky Yuti
Fearon Eric R.
Frost Philip
Pardoll Drew
Vogelstein Bert
Board Of Regents The University Of Texas System
Oyen Wiggs Green & Mutala Llp
The Board Of Regents Of The University Of Texas System
The Johns Hopkins University
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