Methods and compositions for hybrid cell vaccines for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A01N 63/00 (2006.01) A61K 48/00 (2006.01) A01N 65/00 (2006.01)

Patent

CA 2558382

The present invention relates to methods for treating and preventing cancer and for treating precancerous lesions by administering a therapeutically effective dose of a vaccine comprising fusion cells formed by fusion of antigen presenting cells and non-dendritic cells that contain genomic DNA or cDNA derived from a tumor cell or a pre-cancerous cell to a cancer patient or patient with a precancerous lesion. In certain embodiments, such vaccines are administered in combination with a cytokine or other molecule that stimulates a cytotoxic T cell (CTL) response and/or a humoral immune response. The present invention also relates to methods for treating and preventing an infectious disease by administering a therapeutically effective dose of a vaccine comprising fusion cells formed by fusion of antigen presenting cells and non-dendritic cells that contain genomic DNA or cDNA derived from the infectious agent that causes the infectious disease to be treated or prevented to a subject. The present invention also related to methods for producing the fusion cells to be used with the methods of the invention. The present invention also provides compositions comprising the fusion cells to be used with the methods of the invention.

L'invention porte sur des procédés de traitement et de prévention de cancers ou de lésions précancéreuses par administration à un patient cancéreux ou atteint d'une lésion précancéreuse d'une dose à effet thérapeutique d'un vaccin de cellules fusionnées résultant de la fusion de cellules présentant un antigène et de cellules non dendritiques contenant de l'ADN ou de l'ADNc génomique dérivant d'une cellule tumorale ou prétumorale. Dans certaines exécutions de tels vaccins sont administrés en association avec une cytokine ou une autre molécule qui stimule la réponse d'une cellule T cytotoxique et/ou une récepteur immunitaire humorale. L'invention porte également sur des procédés de traitement et prévention d'une maladie infectieuse consistant à administrer à un patient une dose à effet thérapeutique d'un vaccin de cellules fusionnées résultant de la fusion de cellules présentant un antigène et de cellules non dendritiques contenant de l'ADN ou de l'ADNc génomique dérivant de l'agent infectieux ayant causé la maladie infectieuse à traiter ou qu'on souhaite prévenir. L'invention porte en outre sur des compositions comportant lesdites cellules fusionnées.

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