Methods and compositions for identifying streptococcus...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/52 (2006.01) A61K 39/09 (2006.01) C12N 15/57 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01) A61K 38/48 (2006.01) C07K 16/40 (2006.01)

Patent

CA 2199867

Methods and compositions are provided for identifying a group A Streptococcus in any of a variety of physiological samples, such as blood, saliva, throat swabs, cerebrospinal fluid, brocheolar lavage material, and biopsy material. The compositions used include an extracellular cysteine protease or a fragment thereof, obtainable from S. pyogenes and containing at least one conserved epitope, or nucleic acid encoding the cysteine protease or fragment thereof. The compositions also find use in eliciting a protective immune response against a group A Streptococcus infection.

L'invention concerne des procédés et des compositions permettant d'identifier un Streptococcus du groupe A dans divers échantillons physiologiques, tels que du sang, de la salive, des prélèvements dans la gorge, du liquide céphalo-rachidien, des matières de lavage broncho-alvéolaire et de biopsies. Les compositions utilisées comprennent une cystéine protéase extracellulaire ou un fragment de cette dernière, pouvant être obtenu à partir de S. pyogènes et contenant au moins un épitope conservé ou un acide nucléique codant la cystéine protéase ou le fragment de celle-ci. Les compositions peuvent également être utilisées pour induire une réponse immunitaire de protection contre les infections par un Streptocoque du groupe A.

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