Methods and compositions for immunizing against pseudomonas...

A - Human Necessities – 61 – K

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A61K 39/02 (2006.01) C07K 14/21 (2006.01)

Patent

CA 2583226

Methods and compositions for inducing an immune response against Pseudomonas aeruginosa are provided herein. In one aspect, the invention provides a chimeric immunogen, comprising a receptor binding domain, a translocation domain, and a Pseudomonas pilin peptide comprising an amino acid sequence that is TAADGLWKCTSDQDEQFIPKGCSK (SEQ ID NO.: 1), wherein the chimeric immunogen, when administered to a subject, induces an immune response in said subject that is effective to reduce adherence of a microorganism that expresses said Pseudomonas pilin peptide to epithelial cells of said subject. In other aspects, the invention provides nucleic acids encoding chimeric immunogens of the invention, kits comprising chimeric immunogens of the invention, cells expressing chimeric immunogens of the invention, and methods of using chimeric immunogens of the invention.

La présente invention concerne des procédés et des compositions destinées à l'induction d'une réponse immunitaire contre Pseudomonas æruginosa. Dans un aspect, l'invention concerne une chimère immunogène comprenant un domaine de liaison à un récepteur, un domaine de translocation, et la Piline du Pseudomonas, ce peptide comprenant la séquence d'acides aminés TAADGLWKCTSDQDEQFIPKGCSK (SEQ ID NO.: 1). En l'occurrence, la chimère immunogène induit, lorsqu'elle est administrée à un sujet une réponse immunitaire de la part de ce sujet, permettant de réduire l'adhérence d'un micro-organisme exprimant le Piline du Pseudomonas sur des cellules épithéliales de ce sujet. Dans d'autres aspects, l'invention concerne des acides nucléiques codant les chimères immunogènes de l'invention, ces nécessaires comprenant les chimères immunogènes de l'invention, des cellules exprimant les chimères immunogènes de l'invention, et des procédés d'utilisation des chimères immunogènes de l'invention.

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