C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/40 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C12N 9/12 (2006.01)
Patent
CA 2749093
The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
La présente invention porte sur des procédés et des compositions pour améliorer l'efficacité de réactions d'amplification d'acide nucléique. L'invention comprend des polymérases hybrides qui présentent une aptitude au traitement accrue par rapport à des polymérases de type sauvage ainsi qu'une activité exonucléase diminuée. L'invention comprend également des procédés, des compositions et des coffrets pour conduire des réactions de synthèse et d'amplification d'acide nucléique dans lesquelles une amplification non spécifique d'amorces est réduite.
Bursey Evan H.
Cheng Man
Fu Rongdian
Gong Xiao-Song
Sullivan John
Bio-Rad Laboratories Inc.
Fetherstonhaugh & Co.
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