Methods and compositions for improving fidelity in a nucleic...

Details Methods and compositions for improving fidelity in a nucleic... Methods and compositions for improving fidelity in a nucleic...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2599477

The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3' hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide.

La présente invention concerne des procédés et des compositions pour améliorer la fidélité d'une réaction de séquençage par synthèse, par utilisation d'un dérivé nucléotidique qui forme une liaison hydrogène avec un nucléotide complémentaire sur une matrice, mais ne parvient pas à former une liaison phosphodiester avec le groupe 3' hydroxyle d'une amorce dans des conditions par ailleurs compatibles avec une réaction de polymérisation, ce qui permet de bloquer l'incorporation d'un nucléotide mal apparié.

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