C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07D 209/04 (2006.01) C07D 209/14 (2006.01) C07H 19/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12P 17/16 (2006.01)
Patent
CA 2375873
Methods and compositions are disclosed for isolation and propagation of high molecular weight DNA. Large segments of DNA isolated from samples obtained directly from natural sources such as soil, undersea core samples, fresh and salt water, air, and other sources may be used to examine entire gene clusters and to isolate expression products of previously unknown and uncharacterized or even extinct organisms. Isolation and manipulation of larger DNA segments than has been previously possible is taught herein.
L'invention concerne des procédés et des compostions destinés à l'isolation et à la propagation d'ADN de poids moléculaire élevé. On peut utiliser de grands segments d'ADN isolés d'échantillons obtenus directement à partir de sources naturelles telles que le sol, d'échantillons carottés sous la mer, eau douce et eau de mer, air, ainsi que d'autres sources, afin d'examiner des batteries entières de gènes et d'isoler les produits d'expression d'organismes jusque là inconnus ou non caractérisés, voire disparus. L'invention concerne l'isolation et la manipulation de segments d'ADN plus grands que ce qui a été possible par le passé.
August Paul R.
Grossman Trudy H.
Loiacono Kara A.
Lynch Berkley A.
Mcneil Ian
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