Methods and compositions for modifying apolipoprotein b mrna...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/62 (2006.01) A61K 9/127 (2006.01) A61K 38/17 (2006.01) A61M 5/178 (2006.01) A61M 37/00 (2006.01) A61P 3/06 (2006.01) A61P 9/10 (2006.01) C07K 14/155 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 9/12 (2006.01) C12N 9/78 (2006.01) C12N 15/12 (2006.01) C12N 15/49 (2006.01) C12N 15/54 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2439472

Products and methods for modifying apolipoprotein B mRNA editing in vivo, reducing serum LDL levels, and treating or preventing an atherogenic disease or disorder are disclosed. Such methods involve the use of a protein including APOBEC-1 or fragments thereof which can edit mRNA encoding apolipoprotein B. The protein including APOBEC-1 can be taken up by cells in the form of a delivery vehicle, such as a liposome or niosome, or directly as a chimeric protein which includes a first polypeptide that includes a protein transduction domain and a second polypeptide that includes APOBEC-1 or a fragment thereof which can edit mRNA encoding apolipoprotein B.

L'invention concerne des produits et des procédés de modification de l'édition des ARN m de l'apolipoprotéine B in vivo, de réduction des taux de LDL dans le sérum et de traitement ou de prévention de maladies ou de troubles athérogènes. Des tels procédés impliquent l'utilisation d'une protéine contenant une APOBEC-1 ou des fragments de celle-ci, qui peut éditer le codage de l'apolipoprotéine B par les ARN m. Cette protéine contenant une APOBEC-1 peut être reprise par des cellules sous la forme d'un vecteur, tel qu'un liposome ou un niosome, ou directement en tant que protéine chimérique qui contient un premier polypeptide comportant un domaine de transduction de protéines et un deuxième polypeptide comportant une APOBEC-1 ou un fragment de celle-ci qui peut éditer le codage de l'apolipoprotéine B par les ARN m.

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