Methods and compositions for modulating flowering and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C07K 14/00 (2006.01) C12N 15/29 (2006.01)

Patent

CA 2575388

The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence- based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.

L'invention concerne des compositions et des procédés pour moduler la période de floraison chez les plantes, ainsi que des séquences de nucléotides RAP2.7 de maïs, dont la surexpression entraîne une floraison tardive et dont la suppression entraîne une floraison précoce. L'invention concerne également une séquence d'ADN qui agit comme un régulateur/ amplificateur du RAP2.7, appelée VGT1. Ladite séquence ne code pas n'importe quelle protéine, mais agit soit comme un élément d'ARNi soit comme un élément régulateur d'ADN ou d'ARN qui régule directement l'expression des gènes de floraison tel que le RAP2.7 ou cible de manière spécifique l'expression d'autres gènes qui commandent les gènes de floraison tels que les RAP2.7. ledit élément peut être utilisé comme un marqueur à base d'une séquence, ce qui permet d'identifier l'autofécondation ou les hybrides qui ont modifié la maturité. L'invention concerne des procédés pour exprimer lesdites séquences de nucléotides dans une plantes destinées à modifier la maturité et la floraison dans lesdites plantes ainsi que des constructions d'expression, des vecteurs et des cellules et des plantes transformées.

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