Methods and compositions for modulating spermatogenesis

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A01K 67/00 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2331779

The present invention relates to compositions and methods for screening compounds that modulate meiotic progression. In particular, it relates to compositions comprising nucleotides from the mouse 5' regulatory region, and transcriptionally active fragments thereof, that control the expression of a testis-enriched protein, SP-10. Specifically provided are expression vectors, host cells, and transgenic animals, wherein the SP-10 promoter controls the expression of a heterologous gene, e.g., green fluorescent protein. The invention relates to methods for using said vectors, cells, and animals for screening candidate molecules for agonists and antagonists of sperm development and fertilization. Methods for using molecules and compounds identified by the screening assays for the therapeutic treatments are provided.

L'invention concerne des compositions et des techniques qui permettent de cribler des composés modulant le déroulement de la méiose. Elle concerne notamment des compositions qui renferment des nucléotides provenant de la région régulatrice 5' de la souris, ainsi que des fragments de ladite région ayant une activité régulatrice, qui régulent l'expression d'une protéine abondante dans le testicule, la SP-10. De manière spécifique, l'invention concerne des vecteurs d'expression, des cellules hôtes et des animaux transgéniques dans lesquels le promoteur SP-10 régule l'expression d'un gène hétérologue, la protéine verte fluorescente par exemple. L'invention concerne aussi des techniques qui permettent d'utiliser lesdits vecteurs, cellules et animaux, pour cribler des molécules candidates à la recherche d'agonistes et d'antagonistes de la production de spermatozoïdes et de la fécondation, ainsi que des techniques qui permettent d'utiliser à des fins thérapeutiques les molécules et les composés identifiés par lesdits criblages.

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