Methods and compositions for obtaining marker-free...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2653231

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

Procédés et compositions pour l'identification de semence transgénique contenant un trangène visé, mais dépourvu de gène marqueur. L'utilisation d'une séquence d'identification qui donne un phénotype détectable augmente l'efficacité d'analyse permettant d'identifier des semences et des plantes dans lesquelles les séquences transgéniques non liées à un gène visé sont séparées de la séquence codant un gène visé.

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