Methods and compositions for peptide libraries displayed on...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2309543

Methods and compositions for peptides or protein fragments displayed on scaffolds and libraries of sequences encoding peptides or protein fragments displayed on scaffolds that permit the properties of the library to be easily and quantitatively monitored are disclosed. The scaffold is a protein that is capable of emitting light. Thus, analysis of the expression of individual members of the library when they are expressed in cells may be carried out using instruments that can analyse the emitted light, such as a flow sorter (FACS), a spectrophotometer, a microtitre plate reader, a CCD, a fluorescence microscope, or other similar device. This permits screening of the expression library in host cells on a cell-by-cell basis, and enrichment of the library for sequences that have predetermined characteristics.

L'invention concerne des procédés et compositions destinés à des peptides ou fragments protéiniques affichés sur des échafaudages, ainsi qu'à des banques de séquences codant ces peptides ou fragments protéiniques affichés sur des échafaudages, et permettant de surveiller facilement et quantitativement les propriétés de la banque. L'échafaudage est une protéine capable d'émettre de la lumière. Ainsi, il est possible d'exécuter l'analyse de l'expression de membres individuels de la banque, lorsque ces membres sont exprimés dans des cellules, au moyen d'instruments capables d'analyser la lumière émise, comme un trieur de cellules dans un flot continu, un spectrophotomètre, un lecteur de plaques de microtitrage, un dispositif à couplage de charge, un microscope à fluorescence, ou autre dispositif analogue. Cette analyse permet le criblage d'une banque d'expression dans des cellules hôtes, cellule par cellule, et l'enrichissement de la banque en séquences possédant des caractéristiques prédéterminées.

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