Methods and compositions for preparation and use of a herpes...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/57 (2006.01) C07H 21/04 (2006.01) C07K 14/035 (2006.01) C07K 16/08 (2006.01) C12N 9/50 (2006.01) C12N 9/99 (2006.01) C12N 15/11 (2006.01) C12N 15/38 (2006.01) C12N 15/79 (2006.01) C12N 15/869 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/573 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2069460

The present invention relates to the identification and purification of a herpes protease and a nucleic acid segment coding for two proteins. The first protein is the herpes protease which is able to cleave itself and also cleave the second protein. This protease is required for the assembly of the herpes virus capsid, therefore is essential for replication. The second protein has previously been designated as the family of. proteins in viral infected cells, ICP35. The protease and its substrates are encoded by overlapping nucleic acid segments. This invention also relates to a promoter sequence for the second protein. Methods are presented of producing a viral protease, screening a protease inhibitor which may be used in a drug designed for the treatment of herpes disease, methods for treating herpes and other viral infections wherein the virus employs a protease substantially similar to the herpes protease, for capsid production. Methods for detecting herpes infections and other viral infections are also disclosed.

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