C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07K 14/47 (2006.01) C07K 17/00 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2379121
Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.
Cette invention concerne des procédés et des compositions qui permettent de produire des banques d'ADNc pleine longueur. Ces procédés consistent à isoler des premiers brins d'ADNc pleine longueur au moyen d'une protéine de fusion d'un domaine eIF-4E et d'un domaine eIF-4G séparés par un lieur flexible. L'invention concerne également cette nouvelle protéine de fusion utilisée avec les procédés précités ainsi que des acides nucléiques codant pour cette protéine et des cellules hôtes capables d'exprimer ladite protéine. L'invention porte enfin sur des kits d'application des procédés susmentionnés. Cette invention convient pour diverses applications dans lesquelles on utilise des banques d'ADNc pleine longueur.
Guegler Karl
Rose Michael J.
Tan Ruoying
Incyte Genomics Inc.
Smart & Biggar
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