C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01) C12N 15/51 (2006.01)
Patent
CA 2726289
The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consisting of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method.
L'invention concerne un procédé de production de protéine hétérologue dans des cellules hôtes mammaliennes à acide nucléique codant la protéine X de l'hépatite B et la protéine hétérologue, qui comprend les étapes suivantes: croissance de cellules hôtes mammaliennes appartenant au groupe des cellules HKB11, CHO, BHK21, C2C12, et HEK293, croissance de cellules hôtes mammaliennes dans une culture en suspension non adhérente, ou croissance de cellules hôtes mammaliennes qui contiennent un acide nucléique fournissant la protéine 1 de liaison au promoteur X-box (XBP1s) egoxène. Les conditions doivent être telles que les cellules mammaliennes expriment HBx, XBP1s exogène si elle est présente, et la protéine hétérologue. L'invention concerne également des compositions permettant la mise en oeuvre du procédé.
Bauzon Maxine
Harkins Richard N.
Hermiston Terry
Jin Fang
Bayer Healthcare Llc
Mbm Intellectual Property Law Llp
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