Methods and compositions for reducing heparan sulfate...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 31/7088 (2006.01) A61K 38/17 (2006.01) A61K 38/37 (2006.01) A61K 48/00 (2006.01) A61P 7/00 (2006.01) C07K 14/755 (2006.01) C12N 9/96 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2434097

The present invention provides methods of increasing the half-life factor VIII. More specifically, the invention methods of increasing the half-life of factor VIII by substituting amino acids in the A2 domain or in the C2 domain of factor VIII or in both domains. It further provides factor VIII mutants produced by these methods. The invention also provides a method of using receptor-associated protein (RAP) to increase the half-life of factor VIII. The invention also provides polynucleotides encoding the mutant factor VIII, polynucleotides encoding RAP, and methods of treating hemophilia using the polypeptides and polynucleotides of the invention.

La présente invention concerne des méthodes qui permettent d'accroître la demi-vie du facteur VIII et plus spécifiquement des méthodes qui permettent d'allonger la demi-vie du facteur VIII au moyen d'une substitution d'acides aminés dans le domaine A2 ou dans le domaine C2 du facteur VIII ou bien encore dans les deux domaines. Cette invention concerne également des mutants du facteur VIII produits à l'aide de ces méthodes; une méthode d'utilisation d'une protéine associée au récepteur (RAP) pour allonger la demi-vie du facteur VIII; des polynucléotides codant le facteur VIII mutant, des polynucléotides codant RAP et des méthodes de traitement de l'hémophilie à l'aide des polypeptides et des polynucléotides selon l'invention.

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