Methods and compositions for regrowth of cryopreserved...

Details Methods and compositions for regrowth of cryopreserved... Methods and compositions for regrowth of cryopreserved...

A - Human Necessities

01

H

A01H 4/00 (2006.01) A01N 3/00 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2473011

The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.

La présente invention fournit des méthodes permettant la régénération de cellules embryogènes de conifères. Les méthodes comprennent les étapes suivantes : a) mise en contact des cellules embryogènes de conifère cryopréservées avec un milieu de transition liquide; b) culture des cellules embryogènes de conifère dans un milieu de régénération pour obtenir une nouvelle croissance des cellules embryogènes de conifère. De manière générale, les cellules embryogènes de conifère cryopréservées sont laissées en contact avec le milieu de transition liquide pendant moins d'environ 24 heures, par exemple pendant 1 heure.

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