Methods and compositions for stable transgenic plant...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01N 3/00 (2006.01) A61K 38/17 (2006.01) A61K 39/00 (2006.01) A61K 39/39 (2006.01) A61K 39/395 (2006.01) A61P 15/00 (2006.01) C07K 14/245 (2006.01) C07K 14/705 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/36 (2006.01)

Patent

CA 2441699

The present invention provides for processing methods that preserve pharmaceutical proteins expressed in plants. Herein raw plant tissue is reduced to a stable homogenate without significant loss of protein or pharmaceutical potency. The homogenate can be used directly for pharmaceutical purposes without the need to further extract, purify, or precipitate the pharmaceutical protein. The present invention further provides a method of effective immunocontraception for animal and human application. Methods are disclosed for producing transgenic plant or plant cells which express contraceptive proteins, and which can be delivered whole, in part, or after processing, to an animal to cause a contraceptive effect in the target species.

L'invention concerne des méthodes de traitement préservant des protéines pharmaceutiques exprimées dans des plantes. On réduit un tissu végétal brut en un homogénat stable sans perte significative de protéine ou d'activité pharmaceutique. On peut utiliser ledit homogénat directement à des fins pharmaceutiques sans avoir recours à une extraction, une purification, ou une précipitation ultérieure de la protéine pharmaceutique. L'invention concerne également un procédé d'immunocontraception efficace pour une application animale et humaine. L'invention concerne enfin des procédés permettant de produire des plantes transgéniques ou des cellules végétales exprimant des protéines contraceptives, et pouvant être distribuées en totalité, partiellement ou après traitement à un animal afin d'obtenir un effet contraceptif chez des espèces cibles.

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