Methods and compositions for stimulating cd45 and thereby...

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/395 (2006.01) A61K 38/17 (2006.01) A61P 25/28 (2006.01) C07K 16/28 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2428023

A method of inhibiting the negative effects of beta-amyloid in the brain of an animal comprising administering an effective amount of a compound that modulates CD45 activity. This invention also relates to compositions for use in stimulating CD45, and assays for une in finding compounds useful in inhibiting the negative effects of beta-amyloid. An assay for use in identifying compounds that inhibit the negative effects of beta-amyloid comprises (a) contacting immune cells with a predetermined amount of one or more test compounds in the presence of beta-amyloid or one or more peptides derived therefrom, (b) monitoring the amount of one or more inflammatory molecules released by the immune cells, and (c) comparing the amount found in step (b) with another amount found in the absence of the one or more test compounds or using a different predetermined amount the one or more test compounds.

L'invention porte sur un procédé inhibant les effets négatifs du béta-amyloïde dans le cerveau d'un animal consistant à lui administrer une quantité donnée d'un composant modulant l'activité du CD45, sur des compositions stimulant l'activité du CD45 et sur un essai de criblage de composés inhibant les effets négatifs du béta-amyloïde. Ledit essai consiste à: (a) mettre en contact des cellules immunes avec un une quantité donnée d'un ou plusieurs composés d'essai en présence de béta-amyloïde ou d'un ou plusieurs peptides en dérivant; (b) suivre la quantité de la ou des molécules inflammatoires libérée par les cellules immunes; et (c) comparer la quantité trouvée à l'étape (b) à une autre quantité trouvée en l'absence du ou des composés d'essai ou en utilisant une quantité donnée différente du ou des composés d'essai.

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