Methods and compositions for synthesis of long chain...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A23D 9/00 (2006.01) A23K 1/00 (2006.01) A23K 1/16 (2006.01) A23L 1/29 (2006.01) A23L 1/30 (2006.01) A61K 31/20 (2006.01) C11B 1/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2285939

The present invention relates to compositions and methods for preparing polyunsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding fatty acid desaturases, including .DELTA.5-desaturases, .DELTA.6- desaturases and .DELTA.12-desaturases, are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more desaturases. Expression of the desaturases with different substrate specificities in the plant system permit the large scale production of polyunsaturated long chain fatty acids such as docosahexaenoic acid, eicosapentaenoic acid, .alpha.-linolenic acid, gamma-linolenic acid, arachidonic acid and the like for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.

L'invention concerne des compositions et des procédés de préparation d'acides gras polyinsaturés à chaîne longue dans des plantes, des parties de plantes et des cellules végétales, tels que les feuilles, les racines, les fruits et les graines. Des séquences nucléotidiques et des produits de reconstruction codant les désaturases d'acide gras, dont les .DELTA.5-désaturases, les .DELTA.6-désaturases et les .DELTA.12-désaturases, sont utilisés pour la génération de parties de plante, de cellules végétales et de plantes transgéniques qui contiennent et expriment un ou plusieurs transgènes codant une ou plusieurs désaturases. L'expression des désaturases présentant des spécificités différentes dans le système de la plante, permet la production à grande échelle d'acides gras insaturés à chaîne longue, tels que l'acide docosahexanoïque, l'acide eicosapentanoïque, l'acide .alpha.-linoléique, l'acide gamma-linoléique, l'acide arachidonique et similaire pour la modification du profil des acides gras de plantes, de parties de plantes et de tissus. La manipulation des profils des acides gras permet la production de quantités industrielles d'huiles et de produits végétaux nouveaux.

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