Methods and compositions for the detection of a nucleic acid...

Details Methods and compositions for the detection of a nucleic acid... Methods and compositions for the detection of a nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2681937

The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure.

La présente invention concerne des procédés, des compositions et des kits pour générer un signal indicateur de la présence d'une séquence cible d'acides nucléiques dans un échantillon comprenant la formation d'une structure de clivage en incubant un échantillon comprenant une séquence cible d'acides nucléiques avec des oligonucléotides en amont et en aval, et le clivage de la structure de clivage avec une nucléase afin de générer un signal. La présence d'un signal détectable est indicatrice de la présence d'une séquence cible d'acides nucléiques et d'une structure de clivage non invasive.

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