Methods and compositions for the detection of soluble...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2105903

Soluble .beta.-amyloid peptide (.beta.AP) is measured in biological fluids at very low concentrations, typically in the range from 0.1 ng/ml to 10 ng/ml. The measurement of .beta.AP concentrations in animals or conditioned medium from cultured cells can be used for drug screening, where test compounds are administered to the animals or exposed to the cultured cells and the accumulation of .beta.AP in the animal or culture medium observed. It has been found that elevated levels of .beta.AP in body fluids, such as blood and cerebrospinal fluid, is associated with the presence of a .beta.AP-related condition in a patient, such as Alzheimer's Disease. Methods for diagnosing and monitoring .beta.AP-related conditions comprise measuring the levels of .beta.AP in such body fluids from a patient.

La bêta-amyloïde soluble est un peptide mesuré dans les liquides biologiques à des concentrations très faibles, généralement dans l'intervalle situé entre 0,1 ng/ml et 10 ng/ml. La mesure de la concentration de la bête-amyloïde chez les animaux ou dans un milieu de culture conditionné peut servir à analyser des médicaments. Les composés à analyser sont administrés à des animaux ou ajoutés à des cellules en culture, et on mesure l'accumulation de bêta-amyloïde chez l'animal ou dans le milieu de culture. Il a été établi qu'une concentration élevée de bêta- amyloïde dans un liquide biologique comme le sang et le liquide cérébro-spinal, est associée à la présence d'une affection mettant en cause la bêta-amyloïde chez un patient, par exemple la maladie d'Alzheimer. Les méthodes permettant de diagnostiquer et de surveiller les affections mettant en cause la bêta-amyloïde comprennent la mesure de la concentration du peptide dans ce type de liquide biologique prélevé chez un patient.

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