Methods and compositions for the determination of protein...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2335392

The present invention provides libraries of tag dominant-negative elements (TDNE) and methods enabling the identification of specific TDNEs that act as dominant-negative elements on a target protein of interest. The present invention further relates to the use of such TDNEs and dominant-negative elements for the identification of protein-protein interactions, and the determination of a target protein's biological activity and function. Furthermore, the present invention relates to the development of means, including small molecule compounds, for disrupting the target protein's biological function and activity.

La présente invention concerne des banques d'éléments marqueurs négatifs dominants (TDNE), ainsi que des méthodes permettant d'identifier des éléments TDNE spécifiques agissant comme des éléments négatifs dominants sur une protéine cible recherchée. La présente invention concerne également l'utilisation de ces éléments TDNE, et de ces éléments négatifs dominants, pour identifier des interactions protéine-protéine, et pour déterminer l'activité et la fonction biologiques de la protéine cible. La présente invention concerne enfin le développement de moyens, notamment de composés de petites molécules, permettant de rompre l'activité et la fonction biologiques de ladite protéine cible.

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