Methods and compositions for the differentiation of stem cells

Details Methods and compositions for the differentiation of stem cells Methods and compositions for the differentiation of stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 5/0789 (2010.01)

Patent

CA 2753208

The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.

La présente invention porte sur des procédés et des compositions pour la production de cellules progénitrices hématopoïétiques ou de cellules progénitrices endothéliales à partir de cellules souches pluripotentes humaines à l'aide d'un milieu de culture cellulaire défini sans le besoin d'utiliser des cellules nourricières ou du sérum. Dans certains modes de réalisation, la différenciation est accomplie dans des conditions atmosphériques hypoxiques. Le milieu défini de la présente invention peut contenir des facteurs de croissance et un composant de matrice. Les cellules progénitrices hématopoïétiques peuvent être davantage différenciées en lignées cellulaires comprenant des globules rouges, des macrophages, de granulocytes et des mégacariocytes. Les cellules progénitrices endothéliales peuvent être en outre différenciées en cellules endothéliales. L'invention porte également sur des dosages par criblage pour l'identification de substances candidates qui affectent la différenciation des cellules souches pluripotentes en cellules progénitrices.

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