Methods and compositions for the expression of a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01)

Patent

CA 2620002

Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

L'invention porte sur des procédés et compositions favorisant l'expression d'un polynucléotide d'intérêt, lesdites compositions comprennent un domaine amplificateur exposé dans SEQ ID NO: 1, 10, 15, ou 16, et ses variantes et fragments actifs. L'invention porte sur des chimères d'ADN comportant au moins un activateur de transcription comprenant la séquence nucléotidique exposée dans SEQ ID NO:1, 10, 15, or 16 ou une de ses variantes ou fragments actifs fonctionnement lié à un promoteur hétérologue. De telles régions chimères de régulation de la transcription peuvent être fonctionnellement liées à tout polynucléotide d'intérêt. L'invention porte en outre sur des cellules, des plantes, des parties de plantes et du matériau génétique comprenant la chimère d'ADN, et sur des procédés d'utilisation desdites régions, et dans des exécutions particulières, sur des procédés d'expression d'un polynucléotide d'intérêt comprenant par exemple des séquences conférant la tolérance aux herbicides et des procédés de sélection de cellules comportant une chimère d'ADN.

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