C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/04 (2006.01) A01H 4/00 (2006.01) C12N 5/02 (2006.01) C12N 15/01 (2006.01) C12P 1/00 (2006.01) A61K 35/78 (1990.01)
Patent
CA 2069122
- 40 - ABSTRACT Sucessful tissue culture methods have been developed which results in stable, long-term cell lines derived from Taxus explants. Some of the cells lines are habituated, that is, do not require plants hormones as supplements to the nutrient culture medium, for long-term maintenance. The cell lines established by use of the novel methods of the present invention are capable of producing taxol, and tacol-related compounds. These cell lines offer a rapidly repriducible, continuously-available sourse for the production of taxol and taxol-related compounds. This production may be scaled to commercial levels by use of the cell lines in bioreactors. The cell lines provide screening assays for species and cultures of Taxus that are sources of taxol and taxol-related compounds. In addition to obtaining the same compositions as presently directly extracted from
Arteca Richard N.
Wickremesinhe Enaksha R.
Fetherstonhaugh & Co.
Penn Research Corp. (the)
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